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Address - Near Namdev Mandir, Parvati Vihar, Thanesar Town, Kurukshetra-136118
Cloning and Mutation Service
01. Cloning of DNA Fragment into Bacterial and or Mammalian Vectors from cDNA or Genomic DNA Cat # BB-SC10
Template DNA of any desired length will be cloned into a vector of choice. Bio Bharati can provide generic pUC / pET vectors or Bio Bharati developed specialized vectors to generate the desired clone as per requirement. Template DNA will be generated from customer specified sources or can be engineered by fusion of DNA fragments according to the need of the customer. Customer may also provide the specific vectors of their choice. The clone will be confirmed by Sequencing.
Note: Cell lines if required for cloning purpose need to be provided by customer.
02. Mutagenesis to Create Mutants Cat # BB-SM20
PCR-Based method with Pfu Polymerase will be used for the generation of mutants. Insertions / Deletions / Substitution of single/multiple bases can be generated as per need. Customer will provide the template. Bio Bharati will design and synthesize the required primers. Mutations will be confirmed by Sequencing.
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